Entamoeba histolytica


TECHLAB manufactures ELISAs for E. histolytica.

Rapid Test


The  E. HISTOLYTICA QUIK CHEK™ test is a rapid membrane enzyme immunoassay for the qualitative detection of adhesin from Entamoeba histolytica in a single use cassette.

It is intended for use with human fecal specimens from patients with diarrhea or dysentery as an aid in the diagnosis of E. histolytica gastrointestinal infection.

  • Detects pathogenic E. histolytica and does not cross react with non-pathogenic E. dispar
  • Offers alternative to labor-intensive ELISAs and PCRs

Results should be considered in conjunction with the patient history.

Results indicators

  • Proper test performance is indicated by the formation of a blue line at the control side (C) of the Reaction Window.
  • A positive result is indicated by the formation of a blue line at the test side (T) of the Reaction Window.


Microassay Plates


The E. HISTOLYTICA II test is a second generation monoclonal antibody-based ELISA for the rapid detection of Entamoeba histolytica adhesin in fecal specimens. It also indicates the presence of the organism in feces as a diagnostic aid.

It is the only test on the market that specifically detects E. histolytica.



  1. Comparison of PCR, Isoenzyme Analysis, and Antigen Detection for Diagnosis of Entamoeba histolytica Infection
    Journal of Clinical Microbiology, February 1998
  2. Longitudinal Study of Intestinal Entamoeba histolytica Infections in Asymptomatic Adult Carriers
    Journal of Clinical Microbiology, October 2003
  3. Detection and Differentiation of Entamoeba histolytica and Entamoeba dispar Isolates in Clinical Samples by PCR and Enzyme-Linked Immunosorbent Assay
    Journal of Clinical Microbiology, January 2003
  4. A New Rapid Combination Test for Clostridium difficile Antigen and Toxins A and B in Feces – Evaluation of the Diagnosis of Amebic Liver Abscess and Intestinal Infection with the TechLab Entamoeba histolytica II Antigen Detection and Antibody Tests
    Journal of Clinical Microbiology, September 2000
  5. Rapid Diagnosis of Entamoeba Infection by Using Entamoeba and Entamoeba histolytica Stool Antigen Detection Kits
    Journal of Clinical Microbiology, October 1995


The TRI-COMBO PARASITE SCREEN test is an enzyme immunoassay for the simultaneous qualitative detection of Giardia spp., Cryptosporidium spp., and/or E. histolytica antigen in human fecal specimens.

The test is indicated for use as a screen for fecal specimens from patients with gastrointestinal illness including dysentery to aid in the diagnosis of enteric infection resulting from giardiasis, cryptosporidiosis or amebiasis.

The TRI-COMBO PARASITE SCREEN test serves as a screen to rule out negative specimens and reduce the number of specimens that require additional follow-up testing. The test uses monoclonal and polyclonal antibodies to cell-surface antigens of Giardia, Cryptosporidium, and E. histolytica.

  • Ideal screening test for the three most common parasitic causes of diarrhea


TRI-COMBO PARASITE SCREEN Poster Presentations and Publications

  1. Multisite Performance Evaluation of an Enzyme-linked Immunosorbent Assay for Detection ofGiardiaCryptosporidium, and Entamoeba histolytica Antigens in Human Stool
    Christy NC, Hencke JD, Escueta-De Cadiz A, Nazib F, von Thien H, Yagita K, Ligaba S, Haque R, Nozaki T, Tannich E, Herbein JF, Petri WA Jr. J, Clin Microbiol May 2012 50(5):1762-3. Epub 2012 Feb 29
  2. Diagnosis of Multiple Enteric Protozoan Infections by Enzyme-linked Immunosorbent Assay in the Guatemalan Highlands
    den Hartog J, Rosenbaum L, Wood Z, Burt D, Petri WA Jr. Am J, Trop Med Hyg. 2013 Jan;88(1):167-71. doi: 10.4269/ajtmh.2012.12-0142. Epub 2012 Nov 26
  3. Assessment of a New Parasitology Screening Diagnostic ELISA for the Detection of Antigens of Giardia spp., Cryptosporidium spp., and Entamoeba histolytica in Fecal Specimens
    American Society of Tropical Medicine and Hygiene, December 2011, Philadelphia, PA
  4. Evaluation of a Diagnostic Screening ELISA for the Detection of Giardia spp., Cryptosporidium spp. and E. histolytica in Human Fecal Specimen
    American Society of Tropical Medicine and Hygiene, December 2011, Philadelphia, PA
  5. Simultaneous Detection of Entamoeba histolyticaCryptosporidium spp., and Giardia lamblia in Fecal Samples Using a Single Enzyme Immunoassay
    Infectious Diseases Society of America, October 2008, Washington, DC